크로마토그래피 원리의 큰 틀도 마찬가지로 두 상에 대한 분배 차이를 이용하여 분석물을 분리, 정제할 수 있습니다. 다만 크로마토그래피에서 두 개의 상은 하나는 고정하고 다른 하나는 일정 방향으로 이동시켜 사용합니다.
Ion-exchange: Separates charged molecules based on their own interaction with billed useful teams around the stationary period.
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By subsequent these guidelines and systematically addressing prospective causes, you'll be able to efficiently troubleshoot prevalent HPLC complications and be certain your analyses are precise and reputable.
are produced by reacting the silica particles using an organochlorosilane of the final variety Si(CH3)2RCl, wherever R is really an alkyl or substituted alkyl group.
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In liquid–liquid chromatography the stationary stage is really a liquid movie coated over a packing content, normally 3–ten μm porous silica particles. Because the stationary stage might be partly soluble while in the cell stage, it could elute, or bleed with the column after a while.
-hydroxybenzoic acid (PH) with a nonpolar C18 column subject to a utmost Examination time of six min. The shaded spots characterize regions where a separation is impossible, Using the unresolved solutes identified.
加温することが多かったため「オーブン、ヒーター」と称されるが、現在では周辺気温より低温にするための冷却機能が付いている装置も多い。また、周辺気温付近で使用する場合にも冷却機能は一定の効果がある。
High-performance liquid chromatography (HPLC) is a strong analytical strategy for separating and pinpointing components in a combination. Acquiring correct and reliable benefits demands very careful interest to every action with the Examination, from sample click here preparation to info interpretation.
The overarching theory of HPLC is chromatography. It's a technique for separating chemicals centered on their differential interactions that has a stationary period and also a cellular phase.
The pressurized liquid is usually a mix of solvents for instance water, acetonitrile and/or methanol and is referred to as the cell phase.
There are several options for monitoring the chromatogram when utilizing a mass spectrometer since the detector. The most typical system should be to continuously scan the entire mass spectrum and report the full sign for all ions reaching the detector for click here the duration of Each and every scan. This overall ion scan delivers universal detection for all analytes. As seen in Determine 12.five.14
The separation of the person elements during the mixture usually takes location while in the stationary section in the column. As an alternative to the glass column, it is ready in chrome steel.